bacterial tag-encoded flx 454-pyrosequencing  (Pyrosequencing Inc)

Journal: MycoKeys

Article Title: Extensive sampling and high-throughput sequencing reveal Posidoniomyces atricolor gen. et sp. nov. ( Aigialaceae , Pleosporales ) as the dominant root mycobiont of the dominant Mediterranean seagrass Posidonia oceanica

doi: 10.3897/mycokeys.55.35682

Figure Lengend Snippet: The dominant Mediterranean seagrass Posidonia oceanica . a Overall appearance, note dense branched root system of the seagrass (encircled) b Posidonia oceanica growing on an approx. 1.5 m thick layer of matte c typical habitat of the dominant Mediterranean seagrass, note the layer of shed seagrass leaves on the seabed.

Article Snippet: Thus, we characterised P. oceanica root mycobionts using tag-encoded 454-pyrosequencing at 32 localities in the NW Mediterranean Sea (covering the distribution of P. oceanica from its westernmost localities to the boundary between the Western and Eastern Mediterranean basins).

Techniques:

Journal: MycoKeys

Article Title: Extensive sampling and high-throughput sequencing reveal Posidoniomyces atricolor gen. et sp. nov. ( Aigialaceae , Pleosporales ) as the dominant root mycobiont of the dominant Mediterranean seagrass Posidonia oceanica

doi: 10.3897/mycokeys.55.35682

Figure Lengend Snippet: List of the Posidonia oceanica localities sampled in this study.

Article Snippet: Thus, we characterised P. oceanica root mycobionts using tag-encoded 454-pyrosequencing at 32 localities in the NW Mediterranean Sea (covering the distribution of P. oceanica from its westernmost localities to the boundary between the Western and Eastern Mediterranean basins).

Techniques:

Journal: MycoKeys

Article Title: Extensive sampling and high-throughput sequencing reveal Posidoniomyces atricolor gen. et sp. nov. ( Aigialaceae , Pleosporales ) as the dominant root mycobiont of the dominant Mediterranean seagrass Posidonia oceanica

doi: 10.3897/mycokeys.55.35682

Figure Lengend Snippet: List of main MOTUs (with at least 10 sequences) obtained in this study by tag-encoded 454-pyrosequencing.

Article Snippet: Thus, we characterised P. oceanica root mycobionts using tag-encoded 454-pyrosequencing at 32 localities in the NW Mediterranean Sea (covering the distribution of P. oceanica from its westernmost localities to the boundary between the Western and Eastern Mediterranean basins).

Techniques:

Journal: MycoKeys

Article Title: Extensive sampling and high-throughput sequencing reveal Posidoniomyces atricolor gen. et sp. nov. ( Aigialaceae , Pleosporales ) as the dominant root mycobiont of the dominant Mediterranean seagrass Posidonia oceanica

doi: 10.3897/mycokeys.55.35682

Figure Lengend Snippet: In vivo root colonisation pattern and in vitro cultural aspects of Posidoniomyces atricolor . a In vivo colonisation on the root surface (arrows) and in the hypodermis (asterisks) of P. oceanica b DSE colonisation on the root surface c germinating microsclerotia stained with trypan blue (arrows) d compact colony developed from microsclerotia (arrow) e surface-sterilised root segments yielding P. atricolor compact colonies (black arrows), sometimes with substrate mycelium (white arrows) f compact colonial morphotype g mycelial colonial morphotype h mycelial morphotype developing from microsclerotia (arrows) in transversal section. Scale bars: 20 μm ( a, b ), 50 μm ( c ), 100 μm ( d ), 200 μm ( f, h ), 500 μm ( g ).

Article Snippet: Thus, we characterised P. oceanica root mycobionts using tag-encoded 454-pyrosequencing at 32 localities in the NW Mediterranean Sea (covering the distribution of P. oceanica from its westernmost localities to the boundary between the Western and Eastern Mediterranean basins).

Techniques: In Vivo, In Vitro, Staining

Journal: BMC Microbiology

Article Title: Bacterial associates of seed-parasitic wasps (Hymenoptera: Megastigmus )

doi: 10.1186/s12866-014-0224-4

Figure Lengend Snippet: Major bacterial OTUs associated with M. spermotrophus (greater than 0.5% average relative abundance) based on 16S rRNA amplicons from pyrosequencing

Article Snippet: Summary of sequence data from tag encoded FLX 454-pyrosequencing of 16S rRNA from M. spermotrophus , Eurytoma sp. and P. menziesii ovule samples.

Techniques:

Journal: BMC Microbiology

Article Title: Bacterial associates of seed-parasitic wasps (Hymenoptera: Megastigmus )

doi: 10.1186/s12866-014-0224-4

Figure Lengend Snippet: Maximum likelihood phylogeny for Ralstonia 16S rRNA sequence constructed using the Tamura-Nei with invariant sites and gamma distributed rate among sites model of nucleotide substitution. Numbers next to the nodes indicate percentage of bootstrap support from 500 bootstrap replicates. Nodes without numbers received less than 65% bootstrap support.

Article Snippet: Summary of sequence data from tag encoded FLX 454-pyrosequencing of 16S rRNA from M. spermotrophus , Eurytoma sp. and P. menziesii ovule samples.

Techniques: Sequencing, Construct